|Year : 2017 | Volume
| Issue : 1 | Page : 43-53
Can vitamin E protect the pancreas against ethanol-induced alterations in adult male albino rats? A histomorphometric and immunohistochemical study
Ayman A El-Enein Rizk MD 1, Shereen Abdel Fattah1, Mohamed El Sebaie2
1 Department of Anatomy and Embryology, Faculty of Medicine, Cairo University, Cairo, Egypt
2 Department of Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt
|Date of Submission||24-Jan-2017|
|Date of Acceptance||26-Feb-2017|
|Date of Web Publication||29-May-2017|
Ayman A El-Enein Rizk
Department of Anatomy and Embryology, Cairo University, Cairo, 12552
Source of Support: None, Conflict of Interest: None
Ethanol has a destructive effect on the pancreas by inducing oxidative damage on pancreatic cells. This injurious effect could be ameliorated by the administration of vitamin E.
The aim of the study was to investigate the possible protective role of vitamin E on ethanol-induced alterations in the pancreas in adult male albino rats and also the effect of ethanol withdrawal.
Materials and methods
Forty adult male albino rats were divided into four equal groups (10 rats each): group I (control group), group II (ethanol-treated group), group III (ethanol and vitamin E-treated group), and group IV (recovery group). At the end of the experiment, the rats were sacrificed by cervical dislocation and their pancreas were excised and prepared for light microscopic and histochemical study. Detection of interleukin (IL)-1B and IL-6 by real-time PCR and proliferating cell nuclear antigen immunoexpression in the pancreatic tissue was carried out.
Group II showed loss of acinar tissue, distorted degenerated pancreatic acini, dilated distorted interlobular ducts, distorted islets of Langerhans, and dilatation and congestion of the blood vessels. Interlobular inflammatory cellular infiltration was also detected. There was an increased collagen fiber deposition and diminution of the proliferating stem cells. There was a marked increase in IL-1B and IL-6 levels. Group III showed an obvious improvement in the structure of the pancreas. The recovery group showed improvement in the structure of the pancreas and minimal collagen deposition.
Vitamin E could protect the pancreas against ethanol-induced alteration in adult male albino rats.
Keywords: ethanol, interleukin, oxidative stress, pancreas, vitamin E
|How to cite this article:|
El-Enein Rizk AA, Fattah SA, El Sebaie M. Can vitamin E protect the pancreas against ethanol-induced alterations in adult male albino rats? A histomorphometric and immunohistochemical study. Kasr Al Ainy Med J 2017;23:43-53
|How to cite this URL:|
El-Enein Rizk AA, Fattah SA, El Sebaie M. Can vitamin E protect the pancreas against ethanol-induced alterations in adult male albino rats? A histomorphometric and immunohistochemical study. Kasr Al Ainy Med J [serial online] 2017 [cited 2018 May 27];23:43-53. Available from: http://www.kamj.eg.net/text.asp?2017/23/1/43/207194
| Introduction|| |
Chronic alcohol abuse is a major cause of pancreatitis; yet the mechanisms whereby ethanol exerts its toxic effects are not still understood. Prolonged alcohol intake at high doses causes cellular damage. Several researches have elucidated the destructive effects of ethanol on the pancreas and also that excessive alcohol intake is a major contributing factor of pancreatitis. Chronic pancreatitis is a disease characterized by irreversible damage to the pancreatic tissue in the form of fibrosis with progressive loss of both exocrine and endocrine architectures .
The acinar cells produce large amounts of digestive enzymes that in turn cause tissue damage. Ethanol is metabolized by the pancreatic acinar cells and the molecular alterations in these cells could lead to significant cellular injury .
Ethanol causes dose-dependent injury to the pancreatic tissue, characterized by edema, acinar vacuolization, and activation of trypsinogen. Whereas chronic infusion of ethanol causes marked pancreatic edema, inflammatory cellular infiltration, acinar necrosis, and fibrosis .
Pancreatic alcohol-induced fibrosis was assumed to be an active inflammatory process due to the expression of transforming growth factor-β1 and fibrosis-related cytokines produced by the pancreatic cells, accompanied by dynamic signals and cell-to-cell interactions. Pancreatic fibrosis also affects the pancreatic ducts, resulting in their dilatation and formation of pancreatic stones. Obstruction of the main pancreatic duct might lead to acute exacerbation of chronic pancreatitis, which could induce necrosis fibrosis sequence .
Fibrosis results in stricture of the main pancreatic duct, which, in turn, increases the pressure in the pancreatic duct, leading to abdominal pain. In addition to mechanical obstruction, pancreatic fibrosis also causes abdominal pain by affecting the nerves in the pancreas . In addition to the exocrine (acinar cells) pancreas, endocrine pancreas (islet cells) is also affected by fibrosis. Despite this, diabetes only becomes evident at the later stages of the disease .
Alcohol-induced cytotoxicity could be attenuated by antioxidants that could suppress the apoptotic signals induced by other stimuli such as hypoxia, tumor necrosis factor-α (TNF-α), and oxidative stresses including nitric oxide, hydrogen peroxide, and superoxide . Ethanol could induce apoptosis and inhibit pancreatic proliferation through oxidative stress and caspase formation . On the other hand, continued alcohol administration increases pancreatic damage and cell necrosis. Meanwhile, alcohol withdrawal might prevent the progression of pancreatic lesion and apoptosis .
Ethanol administration augments nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity in the pancreatic cells producing reactive oxygen species, explaining the role of ethanol-induced oxidative damage in the pancreatic cells. These injurious effects could be ameliorated by antioxidant administration assuring the role of antioxidants including vitamin E and N-acetyl-cysteine in inhibiting ethanol-induced injury . Vitamin E plays a leading role in controlling excessive oxidative radical formation in cellular membranes including mitochondrial membranes .
The aim of the present study was to investigate the possible protective role of vitamin E on ethanol-induced alterations in the pancreas in adult male albino rats and also the effect of alcohol withdrawal.
| Materials and methods|| |
The present study was carried out on 40 Sprague-Dawley adult male albino rats weighing 200–220 g. They were obtained from the animal house, Faculty of Medicine, Cairo University. The rats were kept in separate cages (five/cage) and were maintained under standard laboratory conditions; diet and water were given ad libitum. The animals were acclimatized in the laboratory for 2 weeks before carrying out the experiment. All the animals were treated in accordance to the international guidelines for the care and use of laboratory animals. All procedures performed in the study were in accordance with the ethical standards of the institution of practice at which the studies were conducted.
- Ethanol (ethanol 50%): It was obtained from the Department of Biochemistry in the form of a glass bottle containing 1 l of 50% concentration of ethanol dissolved in saline (each 100 ml saline contains 50 g of ethanol). Each rat received ethanol (16 g/kg/day) orally through gastric gavage .
- Vitamin E: It was supplied in the form of 400 mg containing capsules obtained from the El Kahira Pharmaceutical Company. Vitamin E capsules were dissolved in olive oil to obtain a concentration of 4000 mg/100 ml (each 1 ml containing 40 mg of vitamin E). Each rat was administered vitamin E (600 mg/kg/day) orally through gastric gavage .
The rats were divided in to four equal groups (10 rats each):
- Group I (control group).
- Group II (ethanol-treated group), in which each rat received an oral dose of 50% ethanol dissolved in saline (16 g/kg/day)  orally through gastric gavage daily for 4 weeks.
- Group III (ethanol and vitamin E-treated group), in which each rat received an oral dose of 50% ethanol dissolved in saline (16 g/kg/day)  and vitamin E (600 mg/kg/day)  dissolved in olive oil daily for 4 weeks.
- Group IV (recovery group), in which each rat received an oral dose of 50% ethanol dissolved in saline (16 g/kg/day) by gastric gavage daily for 4 weeks, and then the animals were left to survive for another 4 weeks.
At the end of each experimental period, the rats were sacrificed by cervical dislocation. The abdomen of each rat was incised and the pancreas was excised and exposed by a pair of scissors; thereafter, the pancreas was washed with saline, and then pinned on a wax platform.
Pancreatic sections were fixed in 10% formal saline for 24 h, and then processed, after which paraffin blocks were prepared. Paraffin sections of 5 μm were prepared and stained with hematoxylin and eosin for routine histological examination, and with Masson’s trichrome for the detection of collagen fiber deposition . The sections were subjected to a light microscopic examination, carried out in the Histology Department, Faculty of Medicine, Cairo University.
After deparaffinization, and rehydration and blocking of peroxidase, antigen retrieval was performed and sections in the peroxidase substrate solution were incubated overnight with primary antibodies against proliferating cell nuclear antigen (PCNA). Sections were deparaffinized, incubated, cooled, and then treated with 3% hydrogen peroxide solution, and then endogenous peroxide was inhibited. The material had been blocked with 5% BSA in PBS buffer (138 mmol/l NaCl, 30 mmol/l Na2HPO4, 2 mmol/l KH2PO4, pH 7.3) for 10 min and incubated with primary PCNA antibody (PC10; Abcam, United Kingdom) diluted in PBS buffer with 1% BSA (1 : 100 proportion) for 1 h. Horseradish peroxidase conjugated antimouse secondary antibodies (PC10; Abcam) were used for detection. Incubation was carried out for 30 min, followed by detection of antigens was carried out with 3,3′-diaminobenzidine (Sigma-Aldrich Chemical Co., Ltd, Saint Louis, Missouri, USA) used as chromogen. After immunohistochemical reaction, the sections were stained with hematoxylin, were dehydrated, immersed in xylene, and mounted. Positive results were obtained as deeply stained brown nuclei in the affected cells .
Image analyzer analysis
Mean area% of PCNA immunoexpression in the pancreatic cells:
The data were obtained by using the Leica Qwin 500 image analyzer computer system (Leica Qwin, England). The image analyzer consisted of a colored video camera, colored monitor, and a disc of IMB personal computer connected to the microscope, and was controlled by the Leica Qwin 500 software. The image analyzer was first calibrated automatically to convert the measurement units (pixels) produced by the image analyzer program into actual micrometer units. Using the measuring field menu, the area, area%, and standard measuring frame were calculated. Measurements were carried out using an objective lens. Ten readings were obtained for each specimen.
Mean area% of collagen fiber deposition in the pancreatic tissue in Masson’s trichrome-stained sections in all experimental groups:
Using the measuring field menu, the area, area%, and standard measuring frame of a standard area were calculated. In each chosen field the pancreatic tissue was enclosed inside the standard measuring frame and then the connective tissue (CT) area was masked by a blue binary color to be measured. The total area of collagen deposition in each specimen was measured and their mean values for each group were obtained.
Antioxidant enzymes analysis in pancreatic homogenate
Measurement of reduced glutathione (GSH) assay: GSH was estimated using the assay kit (Calbiochem, US) according to the manufacturer’s instructions. The pancreatic homogenates were deproteinized in 5% metaphosphoric acid, and then centrifuged; the GSH contents of the supernatants were measured by the rate of colorimetric change of 5, 5′-dithiobis (nitrobenzoic acid) at 412 nm in the presence of GSH reductase and NADPH.
Real-time polymerase chain reaction study
Detection of interleukin (IL)-1b and IL-6 gene expression by real-time PCR:
- Isolation of pancreatic cells.
- RNA extraction: total RNA was extracted from the cell pellet using SV Total RNA Isolation System Kit (catalog no. Z3100; Promega, Madison, Wisconsin, USA).
- Complementary DNA synthesis: the extracted RNA was reverse transcribed into optical density using AMV Reverse Transcriptase (catalog no. M5101; Promega).
- Quantitative real-time PCR: the gene-specific forward and reverse primer pair was normalized. Each primer (forward and reverse) concentration in the mixture was 5 pmol/μl.
- 50°C 2 min, 1 cycle.
- 95°C 10 min, 1 cycle.
- 95°C 15 s.
- 60°C, 30 s.
- 72°C 30 s, for 40 cycles.
- 72°C 10 min, 1 cycle.
A real-time PCR reaction mixture was 50 μl.
The following mixture was prepared in each optical tube:
- 25 μl SYBR Green Mix (2×).
- 0.5 μl cDNA.
- 2 μl primer pair mix (5 pmol/μl each primer).
- 22.5 μl H2O.
- Data analysis: at the end of a quantitative real-time PCR running with SYBR Green chemistry, the relative quantification was assessed using the Applied Biosystem software (Applied Biosystem, US).
Data were coded and entered using the statistical package SPSS, version 23 (US). Data were summarized using mean and SD for quantitative variables. Comparisons between the experimental groups were carried out using analysis of variance . P-values of less than or equal to 0.05 were considered as statistically significant and less than or equal to 0.001 was considered high significant. All the resultant data were tabulated and presented in graphs.
| Results|| |
Group I (control group)
The pancreatic sections of group I revealed normal pancreatic acini and lightly stained Islets of Langerhans More Details ([Figure 1]a). The pancreatic acini were formed of deeply stained acinar cells. The islets of Langerhans were formed as lightly stained small cells with pale cytoplasm (arrows) ([Figure 1]b), interlobular duct lined by columnar cells ([Figure 1]c). The rat pancreas revealed minimal collagen fiber deposition ([Figure 2]a) and exhibited strong positive immunoexpression of PCNA reaction ([Figure 3]a).
|Figure 1 Photomicrographs of sections in rat pancreas of Group I (a) normal pancreatic acini (A) and islets of Langerhans (IL) (H&E ×100) (b) pancreatic acini (A) are formed of deeply stained acinar cells. Islets of Langerhans (IL) are formed of small cells with pale cytoplasm (arrows) (H&Ex400) (c) interlobular duct(D) (H&E ×400) Group II (d) distorted pancreatic acini (A) vacuolation of acinar cells (arrows) distorted islets of Langerhans (IL) (H&E ×400) (e) dilated interlobular ducts (D) interlobular inflammatory infiltration (arrow) (H&E ×400) (f) dilated congested blood vessels (BV) (H&E ×400)|
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|Figure 2 Photomicrographs of sections in rat pancreas of Group I showing: (a) normal pancreatic acini (A) and islets of Langerhans (IL) (H&E ×100) (b) pancreatic acini (A) are formed of deeply stained acinar cells. Islets of Langerhans (IL) are formed of small cells with pale cytoplasm (arrows) (H&E ×400) (c) interlobular duct(D) (H&E ×400) Group II (d) distorted pancreatic acini (A) vacuolation of acinar cells (arrows) distorted islets of Langerhans (IL) (H&E ×400) (e) dilated interlobular ducts (D) interlobular inflammatory infiltration (arrow) (H&E ×400) (f) dilated congested blood vessels (BV) (H&E ×400).|
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|Figure 3 Photomicrographs of sections of the rat pancreas of group I (a) showing minimal collagen fibers deposition (arrows). Group II (b) showing increased fibrous tissue deposition (arrows). Group III (c) showing minimal collage fiber deposition arrows. Group IV (d) showing moderate increase in collagen fibers deposition (arrows) (Masson’trichrome × 400).|
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Group II (ethanol-treated group)
Group II showed an obvious damage of the pancreatic acinar architecture: distorted degenerated pancreatic acini, vacuolation of the acinar cells (arrows), distorted islets of Langerhans ([Figure 1]d), dilated distorted interlobular ducts, interlobular inflammatory infiltration ([Figure 1]e), and dilated congested blood vessels ([Figure 1]f). The rat pancreas revealed increased fibrous tissue deposition ([Figure 2]b) and exhibited mild positive immunoexpression PCNA reaction ([Figure 3]b).
Group III (vitamin E and ethanol-treated group)
Group III revealed preserved normal pancreatic architecture, pancreatic acini interlobular ducts ([Figure 4]a), and islet cells of Langerhans ([Figure 4]b). The pancreatic sections demonstrated minimal collagen fiber deposition arrows ([Figure 2]c) and exhibited strong positive immunoexpression PCNA reaction ([Figure 3]c).
|Figure 4 Photomicrographs of sections of the pancreatic tissue of Group I (a) exhibiting strong positive immunoexpression of PCNA reaction (arrows). Group II (b) exhibiting mild positive immunoexpression PCNA reaction (arrows). Group III (c) exhibiting strong positive immunoexpression PCNA reaction (arrows). Group IV(d) exhibiting strong positive immunoexpression PCNA reaction (arrows) (×400).|
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Group IV (recovery group)
Sections of the rat pancreas of group IV showed normal pancreatic acini, mild congested dilated blood vessels ([Figure 4]c), normally appeared islet cells of Langerhans, and dilatation of the interlobular ducts ([Figure 4]d). The pancreatic sections revealed moderate increase in collagen fiber deposition ([Figure 2]d) and exhibited strong positive immunoexpression PCNA reaction ([Figure 3]d).
Statistical study of the mean area% of proliferating cell nuclear antigen
Histomorphometric analysis of the mean area percent of PCNA immune-expression in the pancreatic tissue in group II revealed a marked decrease in the mean area percentage of PCNA immune-expression (13.11±1.50%), which was statistically highly significant compared with the corresponding value in group I (30.14±2.67%). However, group III revealed an increase in the mean area% of PCNA immune-expression (26.27±1.114%), which was statistically insignificant compared with the same value in group I, and highly statistically significant as compared with group II. On the other hand, group IV showed reduction in the mean area% (18.13±2.004%), which was statistically significant as compared with the values of group I and group IV ([Table 1] and [Figure 5]).
|Table 1 Mean area% proliferating cell nuclear antigen immunoreactivity of the pancreatic tissue|
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|Figure 5 Showing the mean area % of PCNA immunoreactivity of the pancreatic tissue of all the experimental groups.|
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The mean% of collagen fiber deposition in the pancreatic tissue
Histomorphometric analysis showed marked increase in the mean area of collagen deposition in the pancreatic tissue in group II (27.84±2.53%), which was statistically highly significant compared with the mean value of group I (8.62±0.36%). Meanwhile, group III revealed reversal of the value to nearly normal value (9.15±0.21%), which was statistically insignificant compared with group I. On the other hand, group IV showed increase in the mean area% of collagen fibers (12.42±3.72%), which was statistically significant as compared with the control group (8.62±0.36%) ([Table 2] and [Figure 6]).
|Table 2 Mean area% of collagen fibers deposition of all experimental groups|
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|Figure 6 Showing the mean area % of collagen fibers deposition in the pancreatic tissue of all the experimental groups.|
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Histochemical analysis (glutathione reductase enzyme)
The pancreatic homogenate showed that the mean value of GSH content in the pancreatic homogenate of group II was markedly decreased (0.06±0.02 nmol/mg protein), which was statistically highly significant as compared with the corresponding value of group I (0.34±0.14 nmol/mg protein). However, group III showed marked increase in GSH content (0.72±0.34 nmol/mg protein), which was statistically highly significant as compared with group I. Meanwhile, group IV showed reduction of GSH concentration (0.23±0.04 nmol/mg protein), which was statistically significant as compared with the control group ([Table 3] and [Figure 7]).
|Table 3 Mean value of glutathione concentration in of the pancreatic tissue|
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|Figure 7 Showing the mean value of reduced glutathione (GTH) in the pancreatic tissue homogenate of all the experimental groups.|
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Estimation of interleukin-1B and interleukin-6 mean values (real-time polymerase chain reaction study)
Mean value of interleukin-1B
The mean value of IL-1B of group II was markedly increased (92.80±8.29 pg/ml), which was statistically highly significant compared with group I. Meanwhile, vitamin E in group III showed reduction of this parameter to 37.90±8.74 pg/ml, which was statistically insignificant compared with the value in group I. Moreover, group IV showed marked increase in the mean value of IL-1B (60.12±9.77 pg/ml), which was statistically highly significant compared with group I.
Mean value of interleukin-6
The mean value of IL-6 of group II was 75.12±12.08 pg/ml, which was statistically highly significant compared with group I (16.42±4.17 pg/ml). Meanwhile, group III revealed marked reduction of this parameter (36.40±12.73 pg/ml), which was statistically significant as compared with group I (16.42±4.17 pg/ml). On the other hand, group IV showed marked increase in IL-6 value (66.10±8.19 pg/ml), which was statistically significant as compared with group I (16.42±4.17 pg/ml) ([Table 4] and [Figure 8]).
|Table 4 Mean values of interleukin-1B and interleukin-6 of all experimental groups|
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|Figure 8 Showing the mean value of IL-1B and IL-6 in the pancreatic tissue of all the experimental groups.|
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| Discussion|| |
Ethanol-induced cellular damage has been well studied in a variety of tissues including the liver, stomach, kidney, and small intestine. However, ethanol toxicity upon the pancreas was still debatable compared to other organs, although it is one of the principal target organs.
In the present study, group II showed a marked damage and distortion of the pancreatic acini. These findings were in agreement with Lee et al.  who related this acinar damage to the oxidative stress induced by ethanol. They added that ethanol is metabolized by three different pathways: alcohol dehydrogenase, microsomal ethanol oxidation system, and catalase. Each one of these pathways generates free radicals resulting in an imbalance leading to oxidative stress, which, in turn, could lead to peroxidation of the lipid bilayer of the cell membrane, which consecutively disintegrates the membrane.
On the other hand Zakhari , Wu et al. , and Shalbueva et al., attributed the harmful effects of ethanol on the pancreatic acini to a nonoxidative pathway in which ethanol is converted to fatty acid ethyl ester, which has a critical effect on the acinar cells.
Moreover Lerch et al. , attributed this alcoholic damage of the acinar cells to the activation of pancreatic enzymes within the acinar cells, which in turn will be released in the interstitium, and resulted in the autodigestion of the pancreatic tissue. Poxleitner et al.  added that the release of these activated pancreatic enzymes into the circulation resulted in the development of multiple organ dysfunctions occurred.
Group II revealed dilatation of the interlobular ducts, which might be explained by Unal et al.  as a result of the toxic effect of ethanol metabolites, which in turn induces the release of digestive and lysosomal enzymes from the pancreatic acinar cells leading to pancreatic duct obstruction, and limitations of pancreatic regeneration and consequently dilatation of the ducts. These finding might be explained by Rotoli et al.  who reported that ethanol caused increase in the permeability of the main pancreatic ductal epithelium, and paracellular permeability of the pancreatic ductal cell line leading to ductal dilatation.
On the other hand, Braganza et al.  related the ductal dilatation to pancreatic fibrosis of the pancreatic ducts resulting in their dilatation and formation of pancreatic stones, due to inadequate drainage of pancreatic juice.
The present study revealed vacuolation of the cytoplasm of the pancreatic acinar cells in group II. The intracellular vacuoles could be attributed to either lipid accumulation or degranulated zymogen granules deposition within the cytoplasm of acinar and islet cells .
Moreover, group II showed dilatation and congestion of the pancreatic blood vessels, which could be explained by Kim et al.  who attributed that to the toxic effect of ethanol, which was assumed to induce acute edematous pancreatitis, which might be converted into an acute necrotizing hemorrhagic resulting in obvious hemorrhage.
In the present study, group II revealed marked inflammatory cellular infiltration, evidenced by significant increase in the level of pancreatic IL-1B and IL-6. These findings were in agreement with Rossol et al.  and Huang et al.  who attributed this increase to the effect of ethanol, which increased the release of all inflammatory mediators and produced acinar cell-specific inflammatory responses and, consequently, the production of TNFα, IL-1β, IL-6, and IL-10, indicating that pancreatic acinar cells are the major source of proinflammatory and anti-inflammatory cytokines. Moreover, Gorelick  mentioned that ethanol could regulate transcription factors for inflammatory regulation such as nuclear factor κB and activator protein-1, inducing pancreatitis. Meanwhile, Dembele et al.  correlated the infiltration of inflammatory cells to the lymphocytes, which played the key role in the progression of pancreatic damage. Lymphocytes can be activated by gut-derived endotoxin and other stimuli to produce oxidants after alcohol consumption.
Group II revealed marked increase in the collagen deposition in the pancreatic tissue. This finding was in agreement with Yamamoto et al.  who reported that tissue fibrosis might result from an imbalance between its synthesis and degradation. The persistence of the alcoholic injury could lead to an increase in matrix production, resulting in a cumulative accumulation of extracellular matrix components. Moreover, Braganza et al. related the mechanism of fibrous tissue formation to the destruction and damage of the acinar cells, which led to the deposition of type I collagen, which is considered as a characteristic feature of advanced chronic pancreatitis. Meanwhile, Alexander et al.  assumed that necrosis of the pancreatic tissue might result in cellular infiltration, and thus fibrosis could be a result of necrosis. This might be due to premature zymogen activation in the pancreatic acini as a result of recurrent attacks of acute alcoholic pancreatitis, which consequently leads to the development of chronic pancreatitis.
In the present study, group II demonstrated marked diminution of the proliferating stem cells and marked decrease in PCNA immunoreactivity in the pancreatic tissue, which could be explained by the results obtained by Hsu et al.  who attributed that to the effect of ethanol in suppressing cell growth factors and cell cycle proteins. Moreover, Ewa et al.  related the suppressed proliferative activity to the reduction in GSH levels, indicating the correlation between the oxidative stress and the suppression of PCNA. Meanwhile, Dembele et al.  attributed that to the toxic effect of ethanol, which inducted β-cell apoptosis and suppression of regeneration of the pancreatic cells, which might lead to mitochondrial dysfunction and oxidative stress.
Group II revealed marked reduction in the value of GSH in the pancreatic tissue, which was in agreement with the findings of Grattagliano et al.  who reported that ethanol caused distortion of the mitochondrial function and mitochondrial respiration, resulting in reduction in ATP synthesis and inhibition of mitochondria’s ability to import GSH needed to protect against the oxidative damage. Thus, ethanol overall decreased the expression of cytosolic antioxidant enzymes including manganese superoxide dismutase, catalase, and GSH.
The pancreatic tissue of group III revealed an obvious improvement and preservation of the pancreatic acini, interlobular duct, and islet of Langerhans. These findings were in agreement with Masamune et al.  who reported that ethanol administration augments NADPH oxidase enzyme activity in the pancreatic cells producing reactive oxygen species, explaining the role of ethanol-induced oxidative damage in the pancreatic cells. Antioxidant administration could ameliorate such injurious effects and assuring the role of antioxidants intake including vitamin E and N-acetyl-cysteine in inhibiting ethanol-induced injury.
Improvement of the pancreatic structure was clear in group III, which was supported by Gomez et al.  who attributed that to the protective effect of vitamin E, which suppresses the oxidative stress induced by ethanol and thus protecting the pancreatic cells from free radicals production and enhancing cellular proliferation and regeneration. Moreover, Erejuwa  added that vitamin E was assumed to increase the level of the natural antioxidants produced by the body, which are needed in natural defensive mechanisms against free radicals and protection from oxidative stress. Carrasco et al.  added that antioxidant (including vitamin E) could prevent the pancreas from oxidative process, lipid peroxidation, and protein oxidation, and enhance the production of antioxidant enzymes. Moreover, Jung et al.  and Gong et al.  assumed that antioxidants could inhibit the apoptotic pathways stimulated by other harmful stimuli such as hypoxia, TNF-α, and oxidative stresses including nitric oxide, hydrogen peroxide, and superoxide. Furthermore, Oruc et al.  confirmed the therapeutic role of antioxidants in the suppression of the inflammatory cytokines in animals with necrotizing pancreatitis.
In the present study, group III showed marked reduction of IL-1B and IL-6 levels in the pancreatic tissue. These findings were supported with Szabo et al.  and Jha et al.  who mentioned that antioxidants are beneficial in inhibition of nuclear factor κB activity as well as reduction of the proinflammatory cytokines such as TNF-α, IL-6, and IL-1, which might play the active role in ethanol-induced oxidative stress in the pancreas.
In the present study, group IV showed reversal of the changes in the pancreatic tissue and improvement of the histological picture of the pancreatic acini, interlobular ducts, and islets of Langerhans. There were minimal fibrous tissue deposition, increase in expression of the proliferating stem and GSH reductase in the pancreatic cells, and reduction of the inflammatory mediators (IL-1B and IL-6). These findings could be explained by Vonlaufen et al.  who attributed the recovery to the fact that alcohol withdrawal could minimize the cellular injury and cell death, and interrupt the fibrogenic process. On the other hand, Muller-Pillasch et al.  assumed that during the recovery period, there was an increase in the production of profibrogenic cytokines, matrix components, and also matrix metalloproteinases. They also reported that the final accumulation of collagen depends on the imbalance between its synthesis and degradation. They added that fibrous tissue formation depends on maintenance of the injury, which could lead to more cellular damage and excess collagen production and fibrosis. Therefore, cessation of the injury could inhibit the whole process and tissue damage .
It could be concluded that vitamin E could protect the pancreas against ethanol-induced alterations in adult male albino rats. The present study also assumed that this pancreatic injury could be reversible after cessation of ethanol administration.
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Conflicts of interest
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[Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5], [Figure 6], [Figure 7], [Figure 8]
[Table 1], [Table 2], [Table 3], [Table 4]