Year : 2017 | Volume
: 23 | Issue : 3 | Page : 141--147
Immunohistochemical study of the expression of Oct-4 in bladder urothelial carcinoma
Amal Asar1, Samia Gabal2, Noha Helmy1, Sara E Khalifa2
1 Department of Histopathology, Medical Division, National Research Center, Cairo, Egypt
2 Department of Pathology, Faculty of Medicine, Cairo University, Cairo, Egypt
A cancer stem cell model was proposed for bladder carcinoma, as there is a subpopulation of tumor cells capable of resisting conventional therapies and surviving treatment to facilitate recurrence and metastasis. Oct-4 is a pluripotency marker of stem cells, which has been found to be associated with worse prognosis in multiple somatic tumors.
We aimed to explore the expression of Oct-4 protein in urothelial carcinoma and some of its variants and also determine whether Oct-4 expression and level of expression are associated with the clinicopathological parameters such as age, sex, grade, stage, morphology, and tumor progression.
Patients and methods
We tested 84 urothelial tumor specimens including all grades, stages, and some variants of urothelial carcinoma for the expression of Oct-4. Two sections were prepared per case for histologic evaluation and immunohistochemical staining by Oct-4 monoclonal antibody. The immunostaining was evaluated semiquantitavely through obtaining an H-score for each case.
All cases took up the Oct-4 stain except for low-grade tumors and carcinoma in-situ cases. The highest percentage of positive cases was seen in invasive urothelial carcinoma with variant morphology other than conventional (19.1%). Presence of Oct-4 expression was significantly associated with grade (P=0.03), histopathologic type of urothelial tumor (P<0.001), category (P<0.001), and tumor progression (P=0.001). Conversely, the level of Oct-4 expression among positive cases was not found to be associated with any of the studied parameters.
Our study calls for considering Oct-4 as a novel marker of prognostic significance that could be implemented in target therapies for urothelial carcinoma.
Sara E Khalifa
Department of Pathology, Faculty of Medicine, Cairo University, Kasr El Ainy Street, Cairo 11728
|How to cite this article:|
Asar A, Gabal S, Helmy N, Khalifa SE. Immunohistochemical study of the expression of Oct-4 in bladder urothelial carcinoma.Kasr Al Ainy Med J 2017;23:141-147
|How to cite this URL:|
Asar A, Gabal S, Helmy N, Khalifa SE. Immunohistochemical study of the expression of Oct-4 in bladder urothelial carcinoma. Kasr Al Ainy Med J [serial online] 2017 [cited 2018 Dec 14 ];23:141-147
Available from: http://www.kamj.eg.net/article.asp?issn=1687-4625;year=2017;volume=23;issue=3;spage=141;epage=147;aulast=Asar;type=0